Diagnostic assay for Rickettsia japonica

Emerg Infect Dis. 2009 Dec;15(12):1994-7. doi: 10.3201/eid1512.090252.

Abstract

We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica-related strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Rickettsia / genetics
  • Rickettsia / isolation & purification*
  • Rickettsia Infections / diagnosis