Abstract
We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Biomarkers
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Borrelia burgdorferi / drug effects
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Borrelia burgdorferi / genetics*
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Cloning, Molecular
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial
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Genes, Bacterial
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Genetic Engineering
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Microbial Sensitivity Tests
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Models, Genetic
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Mutagenesis, Site-Directed
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Mutation
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Protein Synthesis Inhibitors / pharmacology
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Ribosomal Proteins / genetics*
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Selection, Genetic
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Sequence Homology, Amino Acid
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Streptomycin / pharmacology*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Biomarkers
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DNA, Bacterial
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Protein Synthesis Inhibitors
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Ribosomal Proteins
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Streptomycin