A nuclear factor requires Zn2+ to bind a regulatory MRE element of the mouse gene encoding metallothionein-1

Gene. 1991 Jan 15;97(2):295-300. doi: 10.1016/0378-1119(91)90066-k.

Abstract

The metal ion requirement of nuclear proteins for binding to the metal regulatory element d(MREd) of the mouse gene encoding metallothionein-1 was investigated using an in vitro exonuclease III footprinting assay. The specific DNA-binding activity of the factor was inactivated by the chelating agents, EDTA and 1,10-phenanthroline. Binding activity was restored by Zn2+, but not by Cd2+. These results show that Zn2+ ions are a required component for specific in vitro DNA binding of the MREd-binding protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cadmium / pharmacology
  • DNA-Binding Proteins / metabolism*
  • Genes
  • Metallothionein / genetics*
  • Mice
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid*
  • Zinc / pharmacology*

Substances

  • DNA-Binding Proteins
  • Cadmium
  • Metallothionein
  • Zinc