Sensitivity of 8 CE (European Community)-approved rapid disposable tests for anti-HIV antibody detection during and after seroconversion

J Virol Methods. 2010 Apr;165(1):105-7. doi: 10.1016/j.jviromet.2009.12.007. Epub 2009 Dec 28.

Abstract

We evaluated the sensitivity for the anti-HIV antibody detection of the 8 rapid disposable tests (RDTs) EC approved in 2008.

Methods: A panel of 100 native serum samples collected from HIV-1 subtype B or non-B, HIV-1 group O or HIV-2-infected patient and 3 commercial seroconversion panels were tested. 50 sera from HIV-negative patients were included to blind reading and RDT specificity.

Results: All native samples HIV-1 subtype B were reactive; an HIV-1 non-B subtype gave false-negative results with 3 of the 8 tests. False-negative results on HIV-1 group O samples were observed with one RDT. All the HIV-2 samples were detected. Seroconversion sample reactivity ranged from 60 to 86.7% according to the tests.

Conclusion: Despite their lower performances relative to ELISA tests during the HIV seroconversion period, RDT may be of interest in case of chronic infection.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Clinical Laboratory Techniques / methods*
  • European Union
  • False Negative Reactions
  • HIV Antibodies / blood*
  • HIV Infections / diagnosis*
  • HIV Seropositivity
  • HIV-1 / immunology*
  • HIV-2 / immunology*
  • Humans
  • Immunoassay / methods
  • Sensitivity and Specificity

Substances

  • HIV Antibodies