Abstract
Bovine pancreatic ribonuclease A (RNase A) was crystallized from a mixture of small molecules containing basic fuchsin, tobramycin and uridine 5'-monophosphate (U5P). Solution of the crystal structure revealed that the enzyme was selectively bound to U5P, with the pyrimidine ring of U5P residing in the pyrimidine-binding site at Thr45. The structure was refined to an R factor of 0.197 and an R(free) of 0.253.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Binding Sites
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Cattle
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Crystallography, X-Ray
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Ligands
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Models, Molecular
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Mutation
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Protein Binding
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Protein Structure, Tertiary
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Ribonuclease, Pancreatic / chemistry*
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Ribonuclease, Pancreatic / genetics
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Ribonuclease, Pancreatic / metabolism
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Uridine Monophosphate / chemistry*
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Uridine Monophosphate / metabolism
Substances
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Ligands
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Uridine Monophosphate
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Ribonuclease, Pancreatic