Communication between the AAA+ ring and microtubule-binding domain of dynein

Biochem Cell Biol. 2010 Feb;88(1):15-21. doi: 10.1139/o09-127.

Abstract

Dyneins are microtubule motors, the core of which consists of a ring of AAA+ domains. ATP-driven conformational changes of the AAA+ ring are used to drive the movement of a mechanical element (termed the linker domain) that provides the motor's powerstroke and to change the affinity of the motor for microtubules (strong binding during the power stroke and weak binding to allow stepping and recocking of the linker domain). Dynein's microtubule-binding domain (MTBD) is located at the end of a 10 nm long anti-parallel coiled coil (the stalk) and conformational changes that alter the affinity for microtubules must propagate through this coiled coil. A recent crystal structure of dynein's MTBD sheds new light on how this long-range communication along a coiled coil might occur.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Binding Sites / genetics
  • Crystallography, X-Ray
  • Dyneins / chemistry*
  • Dyneins / metabolism*
  • Forecasting
  • Microtubules / genetics
  • Microtubules / metabolism
  • Models, Biological
  • Models, Molecular
  • Molecular Motor Proteins / genetics
  • Molecular Sequence Data
  • Protein Binding / genetics
  • Protein Conformation
  • Protein Structure, Tertiary / genetics

Substances

  • Molecular Motor Proteins
  • Dyneins