Circulating nucleic acids in cancer and pregnancy

Methods. 2010 Apr;50(4):302-7. doi: 10.1016/j.ymeth.2010.02.004. Epub 2010 Feb 8.

Abstract

Circulating nucleic acids are present in the blood of humans and other vertebrates. During the last 10 years researchers actively studied cell-free nucleic acids present in plasma or serum with great expectations of their use as potential biomarkers for cancer and other pathologic conditions. In the present manuscript the main findings related to the principal characteristics of circulating nucleic acids, the hypothesis on their origin and some methodological considerations on sample collection and extraction as well as on some innovative assay methods have been summarized. Recent reports on the importance of circulating nucleic acids in the intercellular exchange of genetic information between eukaryotic cells have been reviewed.

Publication types

  • Review

MeSH terms

  • Apoptosis / genetics
  • Biomarkers
  • DNA / isolation & purification
  • DNA, Neoplasm / blood
  • DNA, Neoplasm / isolation & purification
  • Female
  • Humans
  • Neoplasms / blood*
  • Nucleic Acids / blood*
  • Pregnancy / blood*
  • RNA / isolation & purification
  • RNA, Neoplasm / blood

Substances

  • Biomarkers
  • DNA, Neoplasm
  • Nucleic Acids
  • RNA, Neoplasm
  • RNA
  • DNA