IL-17 contributes to angiogenesis in rheumatoid arthritis

J Immunol. 2010 Mar 15;184(6):3233-41. doi: 10.4049/jimmunol.0903271. Epub 2010 Feb 19.

Abstract

Angiogenesis is an early and a critical event in the pathogenesis of rheumatoid arthritis (RA). Neovascularization is dependent on endothelial cell activation, migration and proliferation, and inhibition of angiogenesis may provide a novel therapeutic approach in RA. In this study, we document a novel role of IL-17 in mediating angiogenesis. Local expression of IL-17 in mouse ankles increases vascularity. We further demonstrate that IL-17 is angiogenic by showing its ability to promote blood vessel growth in Matrigel plugs in vivo. Additionally, IL-17, in concentrations present in the RA joint, induces human lung microvascular endothelial cell (HMVEC) migration mediated through the PI3K/AKT1 pathway. Furthermore, suppression of the PI3K pathway markedly reduces IL-17-induced tube formation. We also show that both IL-17-induced HMVEC chemotaxis and tube formation are mediated primarily through IL-17 receptor C. Neutralization of either IL-17 in RA synovial fluids or IL-17 receptor C on HMVECs significantly reduces the induction of HMVEC migration by RA synovial fluid. Finally, RA synovial fluid immunoneutralized with anti-IL-17 and antivascular endothelial growth factor does not reduce HMVEC migration beyond the effect detected by immunodepleting each factor alone. These observations identify a novel function for IL-17 as an angiogenic mediator in RA, supporting IL-17 as a therapeutic target in RA.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenic Proteins / antagonists & inhibitors
  • Angiogenic Proteins / biosynthesis
  • Angiogenic Proteins / physiology*
  • Animals
  • Ankle Joint / blood supply
  • Ankle Joint / immunology
  • Ankle Joint / pathology
  • Arthritis, Experimental / immunology*
  • Arthritis, Experimental / pathology
  • Arthritis, Experimental / physiopathology*
  • Arthritis, Rheumatoid / immunology*
  • Arthritis, Rheumatoid / pathology
  • Arthritis, Rheumatoid / physiopathology*
  • Cell Line
  • Cell Migration Inhibition / immunology
  • Cell Movement / immunology
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology
  • Endothelium, Vascular / metabolism
  • Humans
  • Inflammation Mediators / antagonists & inhibitors
  • Inflammation Mediators / metabolism
  • Inflammation Mediators / physiology
  • Interleukin-17 / antagonists & inhibitors
  • Interleukin-17 / biosynthesis
  • Interleukin-17 / physiology*
  • Lung / blood supply
  • Lung / immunology
  • Lung / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Interleukin-17 / physiology
  • Synovial Fluid / cytology
  • Synovial Fluid / immunology
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors
  • Vascular Endothelial Growth Factor A / physiology

Substances

  • Angiogenic Proteins
  • IL17RA protein, human
  • Inflammation Mediators
  • Interleukin-17
  • Receptors, Interleukin-17
  • Vascular Endothelial Growth Factor A