Characterization of canine oral papillomavirus by histopathological and genetic analysis in Korea

J Vet Sci. 2010 Mar;11(1):21-5. doi: 10.4142/jvs.2010.11.1.21.

Abstract

In August 2008, forty dogs out of 400 developed oral warts in a breeding farm in Korea. Canine oral papilloma infection is a common disease in dogs. However, there has been no report of an outbreak of canine oral papillomavirus (COPV) in a group of dogs or in dog breeding farms in Korea, and the genetic analysis of COPV in Korea has yet to be performed. This study diagnosed canine oral papilloma from the oral samples of these dogs based on histopathological examination and immunohistochemistry. Polymerase chain reaction was applied to amplify the corresponding products using preexisting primer sets for COPV and a universal human papillomavirus targeting L1 gene. Further genetic analysis of the major viral capsid gene L1 confirms the sequences of Korean COPV, which shows a close relationship to previously reported COPV. This study describes the histopathological and immunohistochemical characteristics of canine oral papilloma in a group of breeding dogs in Korea and discloses the complete L1 gene sequences of Korean COPV.

MeSH terms

  • Animals
  • Base Sequence
  • Capsid Proteins / chemistry
  • Capsid Proteins / genetics
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Disease Outbreaks / veterinary*
  • Dog Diseases / epidemiology
  • Dog Diseases / virology*
  • Dogs
  • Immunohistochemistry / veterinary
  • Korea / epidemiology
  • Lambdapapillomavirus / genetics
  • Lambdapapillomavirus / isolation & purification*
  • Molecular Sequence Data
  • Mouth Diseases / epidemiology
  • Mouth Diseases / veterinary*
  • Mouth Diseases / virology
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / veterinary*
  • Papillomavirus Infections / virology
  • Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA

Substances

  • Capsid Proteins
  • DNA, Viral
  • L1 protein, canine oral papillomavirus

Associated data

  • GENBANK/FJ479789