IFNgamma primes macrophages for inflammatory activation by high molecular weight hyaluronan

Cell Immunol. 2010;262(2):84-8. doi: 10.1016/j.cellimm.2010.02.013. Epub 2010 Feb 24.

Abstract

The objective was to assess outcomes of IFNgamma-priming upon macrophage activation by the synovial macromolecule high molecular weight hyaluronan [HMW-HA] in the context of rheumatoid arthritis inflammation. Human macrophages primed by IFNgamma and activated by HMW-HA were evaluated for cytokine secretion by ELISA and Milliplex assay and activation profiles by nuclear transcription factor EIA. IFNgamma-primed, HMW-HA-activated macrophages produced elevated levels of TNF and secreted the TH1 cytokine IL-12p70, while IFNgamma suppressed HMW-HA-induced secretion of the regulatory cytokine IL-10 and activation of the transcription factor c-Jun. IFNgamma modulates the HMW-HA-induced cytokine response profile promoting macrophage activation and inflammatory TH1 cytokine secretion.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cells, Cultured
  • Cytokines / metabolism
  • Humans
  • Hyaluronic Acid* / chemistry
  • Hyaluronic Acid* / immunology
  • Hyaluronic Acid* / pharmacology
  • Inflammation / immunology*
  • Inflammation / pathology
  • Interferon-gamma* / immunology
  • Interferon-gamma* / pharmacology
  • Joints / cytology
  • Joints / metabolism
  • Joints / pathology
  • Macrophage Activation / drug effects*
  • Macrophages* / drug effects
  • Macrophages* / immunology
  • Molecular Weight
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Cytokines
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Hyaluronic Acid