The use of commercial alcohol products to sterilize bones prior to DNA sampling

Forensic Sci Med Pathol. 2010 Jun;6(2):127-9. doi: 10.1007/s12024-010-9155-z. Epub 2010 Mar 19.

Abstract

In disaster situations it is not always possible to maintain an adequate supply of standard equipment and sterilizing solutions. We have compared bone samples from cadavers cleaned in commercial white alcohol to samples from the same individuals cleaned with 95% surgical spirit. We have found that it is possible to use a commercial, white spirit to clean specimens taken from human cadavers femoral diaphysis collected for DNA analysis.

Publication types

  • Comparative Study

MeSH terms

  • Central Nervous System Depressants*
  • DNA / isolation & purification
  • DNA Fingerprinting*
  • Ethanol*
  • Femur / pathology*
  • Humans
  • Polymerase Chain Reaction
  • Solvents*
  • Specimen Handling
  • Sterilization / methods*

Substances

  • Central Nervous System Depressants
  • Solvents
  • Ethanol
  • DNA