Comparison of rifabutin susceptibility and rpoB mutations in multi-drug-resistant Mycobacterium tuberculosis strains by DNA sequencing and the line probe assay

J Infect Chemother. 2010 Oct;16(5):360-3. doi: 10.1007/s10156-010-0057-5. Epub 2010 Mar 31.

Abstract

We compared rifabutin susceptibility and rpoB mutations in 98 multi-drug-resistant strains of Mycobacterium tuberculosis (MDR-TB) by DNA sequencing and with a line probe assay using the commercially available INNO-LiPA Rif. TB kit (the LiPA). Our results indicated that rifabutin continues to remain active against MDR-TB strains harboring certain genetic alterations and also that the LiPA might be useful in identifying MDR-TB strains susceptible to rifabutin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antitubercular / pharmacology
  • Bacterial Proteins / genetics*
  • DNA Mutational Analysis / methods
  • DNA-Directed RNA Polymerases
  • Drug Resistance, Multiple, Bacterial
  • Humans
  • Microbial Sensitivity Tests / methods
  • Mutation / genetics
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics*
  • Nucleic Acid Hybridization / methods
  • Rifabutin / pharmacology*
  • Tuberculosis, Multidrug-Resistant / genetics*
  • Tuberculosis, Multidrug-Resistant / microbiology*

Substances

  • Antibiotics, Antitubercular
  • Bacterial Proteins
  • rpoB protein, Mycobacterium tuberculosis
  • Rifabutin
  • DNA-Directed RNA Polymerases