A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection

Opt Express. 2010 Mar 15;18(6):5390-8. doi: 10.1364/OE.18.005390.

Abstract

We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Lenses*
  • Microscopy, Fluorescence, Multiphoton / instrumentation*