Human melanoma cells transcribe interleukin 1 genes identical to those of monocytes

Cancer Res. 1991 Jun 15;51(12):3334-5.

Abstract

Two molecular species of the pleotropic cytokine interleukin 1 (IL-1) are produced as products of two distinct genes transcribed by cells of the monocyte-macrophage lineage. We have shown previously that a significant proportion of human melanoma cell lines express IL-1 biological activity, but it has not been demonstrated that this activity is the same as authentic monocyte IL-1 alpha and -beta. Here we report the cloning and sequencing of IL-1 complementary DNAs from a metastatic melanoma cell line and demonstrate that they encode bona fide IL-1 alpha and IL-1 beta. In addition, IL-1 complementary DNAs encoding a different amino acid at position 145 were revealed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Cloning, Molecular
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Gene Library
  • Humans
  • Interleukin-1 / genetics*
  • Melanoma / genetics*
  • Melanoma / immunology
  • Monocytes / immunology*
  • Neoplasm Metastasis
  • Transcription, Genetic*

Substances

  • DNA, Neoplasm
  • Interleukin-1