Comparison of ZFY and ZFX gene structure and analysis of alternative 3' untranslated regions of ZFY

Nucleic Acids Res. 1991 May 25;19(10):2579-86. doi: 10.1093/nar/19.10.2579.

Abstract

We have cloned and sequenced transcripts from the X- and Y-linked zinc-finger genes ZFX and ZFY respectively and discuss a possible mechanism of post-transcriptional control by which these genes can be widely expressed but translated in only specific tissues. We report the identification of a novel 3'UTR (untranslated region) present in ZFY which is highly conserved among primates and contains a series of motifs implicated as mRNA instability determinants. These sequences can be substantially removed by polyadenylation directed from consensus (AATAAA) and non-consensus (AATATAAA) sequences in adult testis. The DNA-binding domains of the ZFY and ZFX proteins are compared using present models for zinc-finger/DNA interactions. Additionally, the genomic organisation of the ZFY coding sequence is presented as compared to that of ZFX.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • Gene Expression Regulation*
  • Humans
  • Introns*
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Zinc Fingers / genetics*

Substances

  • DNA