Abstract
We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription. The assay is robust (Z' factor, >0.6) and can be carried out in low-biosafety containment. Results from a pilot screen of 960 compounds are presented.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antiviral Agents / pharmacology*
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Cell Line
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Ebolavirus / drug effects*
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Ebolavirus / genetics*
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Ebolavirus / physiology
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Genome, Viral / genetics*
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Humans
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Transcription, Genetic / drug effects*
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Transcription, Genetic / genetics
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Virus Replication / drug effects*
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Virus Replication / genetics