We demonstrate the enhancement of the polarization ratio of light emitted from electrospun conjugated polymer nanofibers, by means of nanoimprint lithography carried out at room temperature. We provide evidence of tailoring the polarization properties by patterning light-emitting fibers at the nanoscale. The polarization ratios are increased up to a factor of 2.4 by gratings with periodicity (560 nm) matching the emission wavelength of the employed conjugated polymer. The use of room temperature nanoimprint lithography to pattern light-emitting polymer nanofibers represents a strategic route for realizing photonic crystals and distributed feedback polarized emitters on one-dimensional organic nanostructures.