Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells

Nat Cell Biol. 2010 Jun;12(6):543-52. doi: 10.1038/ncb2056. Epub 2010 May 9.

Abstract

The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in comparison with that of protein transport. We have established a mammalian system that allowed the real-time visualization and quantification of large single mRNA-protein complexes (mRNPs) during export. The in vivo dynamics of bulk mRNP transport and export, from transcription to the nuclear pore complex (NPC), occurred within a 5-40 minute time frame, with no NPC pile-up. mRNP export was rapid (about 0.5 s) and kinetically faster than nucleoplasmic diffusion. Export inhibition demonstrated that mRNA-NPC interactions were independent of ongoing export. Nucleoplasmic transport dynamics of intron-containing and intronless mRNAs were similar, yet an intron did increase export efficiency. Here we provide visualization and analysis at the single mRNP level of the various steps in nuclear gene expression and the inter-chromatin tracks through which mRNPs diffuse, and demonstrate the kinetics of mRNP-NPC interactions and translocation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / genetics
  • Animals
  • Biological Transport / genetics
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism*
  • Cells / metabolism*
  • Chromosomes / metabolism
  • Introns
  • Mammals / genetics
  • Mammals / metabolism
  • Nuclear Pore / genetics
  • Nuclear Pore / metabolism*
  • Protein Transport / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Ribonucleoproteins / metabolism*

Substances

  • RNA, Messenger
  • Ribonucleoproteins
  • messenger ribonucleoprotein