Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter

Andreas Zurbriggen; Philipp Schneider; Priska Bähler; Ulrich Baumann; Bernhard Erni

doi:10.1107/S1744309110013102

Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):684-8. doi: 10.1107/S1744309110013102. Epub 2010 May 26.

Authors

Andreas Zurbriggen¹, Philipp Schneider, Priska Bähler, Ulrich Baumann, Bernhard Erni

Affiliation

¹ Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. andreas.zurbriggen@ibc.unibe.ch

Abstract

The glucose-import system of Escherichia coli consists of a hydrophilic EIIA(Glc) subunit and a transmembrane EIICB(Glc) subunit. EIICB(Glc) (UniProt P69786) contains two domains: the transmembrane EIIC(Glc) domain (40.6 kDa) and the cytoplasmic EIIB(Glc) domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICB(Glc) subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Crystallization
Crystallography, X-Ray
Escherichia coli / chemistry*
Gene Expression
Glucose Transport Proteins, Facilitative / chemistry*
Glucose Transport Proteins, Facilitative / genetics
Glucose Transport Proteins, Facilitative / isolation & purification
Molecular Sequence Data
Protein Structure, Tertiary

Substances

Glucose Transport Proteins, Facilitative