Multiple nucleotide substitutions in the Neisseria meningitidis serogroup C ctrA gene cause false-negative detection by real-time PCR

J Clin Microbiol. 2010 Aug;48(8):3016-8. doi: 10.1128/JCM.00103-10. Epub 2010 Jun 2.

Abstract

Two strains of Neisseria meningitidis serogroup C with disparate sequences of ctrA were isolated. The nucleotide substitutions did not alter the corresponding protein sequences, but they impeded the detection of these meningococcal isolates by real-time PCR.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Bacteremia / microbiology*
  • Bacterial Proteins / genetics*
  • DNA Primers / genetics
  • Female
  • Humans
  • Male
  • Meningococcal Infections / microbiology*
  • Molecular Sequence Data
  • Mutation*
  • Neisseria meningitidis, Serogroup C / genetics*
  • Neisseria meningitidis, Serogroup C / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA Primers

Associated data

  • GENBANK/GU391295
  • GENBANK/GU391296