A nationwide survey for prevalence of hepatitis E virus antibody in qualified blood donors in Japan

Vox Sang. 2010 Nov;99(4):307-13. doi: 10.1111/j.1423-0410.2010.01362.x.

Abstract

Background and objectives: In previous studies, we reported the transmission of hepatitis E virus (HEV) by transfusion, and the frequent detection of HEV markers in Japanese blood donors with elevated ALT levels. For the current study, we carried out a nationwide survey of the prevalence of IgG anti-HEV in qualified blood donors throughout Japan.

Materials and methods: The 12,600 samples from qualified blood donors were collected from seven blood centres (1800 per centre) representing nearly all regions of Japan. Samples were from age- and sex-matched blood donors who tested negative for all the current blood screening tests. The samples were screened using the in-house IgG anti-HEV ELISA. Sequentially, the positive samples were tested by the commercial IgG anti-HEV ELISA.

Results: Of 12,600 samples, 431 (3·4%) were regarded as positive for IgG anti-HEV. The prevalence of IgG anti-HEV was higher in eastern Japan (5·6%) than in western Japan (1·8%) (P<0·001), and was also age-dependent and higher in men (3·9%) than in women (2·9%) (P=0·002).

Conclusion: The spread of the domestic infection of HEV was observed in qualified blood donors in Japan. A higher prevalence of IgG anti-HEV was observed in male donors, older donors and in donors residing in eastern Japan. Further studies are necessary to clarify the potential risk of transfusion-transmission of HEV in Japan.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Blood Donors*
  • Data Collection
  • Donor Selection*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Hepatitis Antibodies / blood*
  • Hepatitis E virus*
  • Hepatitis E* / blood
  • Hepatitis E* / epidemiology
  • Humans
  • Immunoglobulin G / blood*
  • Japan / epidemiology
  • Male
  • Prevalence
  • Seroepidemiologic Studies
  • Sex Factors

Substances

  • Hepatitis Antibodies
  • Immunoglobulin G