An electronic bus bar lies in the core of cytochrome bc1

Science. 2010 Jul 23;329(5990):451-4. doi: 10.1126/science.1190899.

Abstract

The ubiquinol-cytochrome c oxidoreductases, central to cellular respiration and photosynthesis, are homodimers. High symmetry has frustrated resolution of whether cross-dimer interactions are functionally important. This has resulted in a proliferation of contradictory models. Here, we duplicated and fused cytochrome b subunits, and then broke symmetry by introducing independent mutations into each monomer. Electrons moved freely within and between monomers, crossing an electron-transfer bridge between two hemes in the core of the dimer. This revealed an H-shaped electron-transfer system that distributes electrons between four quinone oxidation-reduction terminals at the corners of the dimer within the millisecond time scale of enzymatic turnover. Free and unregulated distribution of electrons acts like a molecular-scale bus bar, a design often exploited in electronics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cytochromes c / metabolism
  • Electron Transport Complex III / chemistry*
  • Electron Transport Complex III / genetics
  • Electron Transport Complex III / metabolism*
  • Electron Transport*
  • Electrons*
  • Models, Molecular
  • Mutant Proteins / chemistry
  • Mutant Proteins / metabolism
  • Oxidation-Reduction
  • Point Mutation
  • Protein Conformation
  • Protein Multimerization
  • Protein Subunits / chemistry
  • Protein Subunits / metabolism
  • Rhodobacter capsulatus / enzymology*
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / metabolism

Substances

  • Bacterial Proteins
  • Mutant Proteins
  • Protein Subunits
  • Ubiquinone
  • Cytochromes c
  • Electron Transport Complex III
  • ubiquinol