DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips

Chem Commun (Camb). 2010 Sep 7;46(33):6126-8. doi: 10.1039/c0cc01608j. Epub 2010 Jul 27.

Abstract

A DNA-bridged strategy is used to facilely conjugate streptavidin (STV) to luminescent semiconductor quantum dots (QDs), which leads to convenient and stable QD-DNA-biotin-STV conjugates that serve as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers with a microfluidic protein chip.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Biomarkers, Tumor / analysis
  • Biotin / chemistry*
  • Carcinoembryonic Antigen / analysis
  • DNA / chemistry*
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescence
  • Microfluidic Analytical Techniques / methods*
  • Microscopy, Atomic Force
  • Particle Size
  • Protein Array Analysis / methods*
  • Quantum Dots*
  • Sensitivity and Specificity
  • Streptavidin / chemistry*

Substances

  • Biomarkers, Tumor
  • Carcinoembryonic Antigen
  • Biotin
  • DNA
  • Streptavidin