Follicular B cell trafficking within the spleen actively restricts humoral immune responses

Immunity. 2010 Aug 27;33(2):254-65. doi: 10.1016/j.immuni.2010.07.016. Epub 2010 Aug 5.

Abstract

Follicular (FO) and marginal zone (MZ) B cells are maintained in distinct locations within the spleen, but the genetic basis for this separation is still enigmatic. We now report that B cell sequestration requires lineage-specific regulation of migratory receptors by the transcription factor Klf2. Moreover, using gene-targeted mice we show that altered splenic B cell migration confers a significant in vivo gain-of-function phenotype to FO B cells, including the ability to quickly respond to MZ-associated antigens and pathogens in a T cell-dependent manner. This work demonstrates that in wild-type animals, naive FO B cells are actively removed from the MZ, thus restricting their capacity to respond to blood-borne pathogens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD19 / genetics
  • Antigens, CD19 / immunology
  • Antigens, T-Independent / genetics
  • Antigens, T-Independent / immunology
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology*
  • Bone Marrow / immunology
  • Cell Differentiation
  • Cell Movement*
  • Cells, Cultured
  • Immunity, Humoral*
  • Kruppel-Like Transcription Factors / deficiency
  • Kruppel-Like Transcription Factors / immunology
  • Mice
  • Mice, Knockout
  • Receptors, CCR / immunology
  • Spleen / cytology*
  • Spleen / immunology*

Substances

  • Antigens, CD19
  • Antigens, T-Independent
  • Klf2 protein, mouse
  • Kruppel-Like Transcription Factors
  • Receptors, CCR