Sonothrombolysis of intra-catheter aged venous thrombi using microbubble enhancement and guided three-dimensional ultrasound pulses

J Am Soc Echocardiogr. 2010 Sep;23(9):1001-6. doi: 10.1016/j.echo.2010.06.024. Epub 2010 Aug 8.

Abstract

Background: Central venous and arterial catheters are a major source of thromboembolic disease in children. The investigators hypothesized that guided high-mechanical index (MI) impulses from diagnostic three-dimensional (3D) ultrasound during an intravenous microbubble infusion could dissolve these thrombi.

Methods: An in vitro system simulating intracatheter thrombi was created and then treated with guided high-MI impulses from 3D ultrasound, using low-MI microbubble sensitive imaging pulse sequence schemes to detect the microbubbles. Ten aged thrombi >24 hours old were tested using 3D ultrasound coupled with a continuous diluted microbubble infusion (group A) and 10 with 3D ultrasound alone (group B).

Results: The mean thrombus age was 28.6 hours (range, 26.6-30.3 hours). Group A exhibited a 55 +/- 19% reduction in venous thrombus size compared with 31 +/- 10% in group B (P = .008). Feasibility testing was performed in four pigs, establishing an in vivo model to investigate further the efficacy of this approach.

Conclusions: Sonothrombolysis of aged intracatheter venous thrombi can be achieved with commercially available microbubbles and guided high-MI ultrasound from a diagnostic 3D transducer.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Catheterization, Central Venous / adverse effects*
  • Contrast Media / administration & dosage
  • Disease Models, Animal
  • Equipment Design
  • Feasibility Studies
  • Imaging, Three-Dimensional
  • In Vitro Techniques
  • Infusions, Intravenous
  • Microbubbles
  • Phantoms, Imaging
  • Swine
  • Ultrasonic Therapy / methods*
  • Ultrasonography, Interventional
  • Venous Thrombosis / diagnostic imaging
  • Venous Thrombosis / therapy*

Substances

  • Contrast Media