Peripheral blood cytokine profiling during pregnancy and post-partum periods

Am J Reprod Immunol. 2010 Dec;64(6):411-26. doi: 10.1111/j.1600-0897.2010.00889.x. Epub 2010 Aug 16.

Abstract

Problem: Pregnancy requires that the maternal immune system adapt to prevent rejection of the fetal semi-allograft. This immunologic adaptation may contribute to pregnancy-related alterations in disease susceptibility and severity of infections from viral pathogens such as influenza virus.

Method of study: As part of a larger study investigating the maternal systemic immune response during pregnancy, peripheral blood was collected three times during pregnancy and twice post-partum to measure serum levels of 23 cytokines, chemokines, and growth factors. This longitudinal study design allowed each woman's post-partum blood draw to serve as her own comparison, thus controlling for interpersonal variability in expression levels.

Results: When compared to the post-partum samples, significant pregnancy-related changes in IFNγ, TNFα, VEGF, GCSF, Eotaxin, and MCP-1 expression were observed. These changes have significant immunologic effects in vivo and in culture.

Conclusion: Pregnancy-associated changes to steady state serum cytokines may have important immunologic consequence.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Cells, Cultured
  • Cytokines / genetics
  • Cytokines / metabolism*
  • Female
  • Follow-Up Studies
  • Gene Expression Profiling
  • Gene Expression Regulation / immunology
  • Histocompatibility
  • Humans
  • Immune Tolerance
  • Immunity, Cellular
  • Influenza, Human / immunology*
  • Influenza, Human / physiopathology
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism*
  • Leukocytes, Mononuclear / pathology
  • Leukocytes, Mononuclear / virology
  • Orthomyxoviridae / immunology*
  • Orthomyxoviridae / pathogenicity
  • Postpartum Period / immunology
  • Pregnancy / immunology
  • Pregnancy Complications, Infectious / immunology*
  • Pregnancy Complications, Infectious / physiopathology
  • Th1-Th2 Balance

Substances

  • Cytokines