On the use of 2,1,3-benzothiadiazole derivatives as selective live cell fluorescence imaging probes

Felipe F D Oliveira; Diego C B D Santos; Alexandre A M Lapis; José R Corrêa; Alexandre F Gomes; Fabio C Gozzo; Paulo F Moreira Jr; Virgínia C de Oliveira; Frank H Quina; Brenno A D Neto

doi:10.1016/j.bmcl.2010.08.073

On the use of 2,1,3-benzothiadiazole derivatives as selective live cell fluorescence imaging probes

Bioorg Med Chem Lett. 2010 Oct 15;20(20):6001-7. doi: 10.1016/j.bmcl.2010.08.073. Epub 2010 Aug 20.

Authors

Felipe F D Oliveira¹, Diego C B D Santos, Alexandre A M Lapis, José R Corrêa, Alexandre F Gomes, Fabio C Gozzo, Paulo F Moreira Jr, Virgínia C de Oliveira, Frank H Quina, Brenno A D Neto

Affiliation

¹ Laboratory of Medicinal and Technological Chemistry, University of Brasília (IQ-UnB), Brasília, DF, Brazil.

PMID: 20826088
DOI: 10.1016/j.bmcl.2010.08.073

Abstract

Newly designed 2,1,3-benzothiadiazole-containing fluorescent probes with four excited state intramolecular proton transfer (ESIPT) sites were successfully tested in live cell-imaging assays using a confluent monolayer of human stem-cells (tissue). All tested dyes were compared with the commercially available DAPI and gave far better results.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival
Cells, Cultured
DNA, Single-Stranded / analysis
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemistry
Humans
Spectrometry, Fluorescence
Stem Cells / cytology*
Thiadiazoles / analysis*
Thiadiazoles / chemistry

Substances

DNA, Single-Stranded
Fluorescent Dyes
Thiadiazoles
benzo-1,2,3-thiadiazole