Shedding of large functionally active CD11/CD18 Integrin complexes from leukocyte membranes during synovial inflammation distinguishes three types of arthritis through differential epitope exposure

J Immunol. 2010 Oct 1;185(7):4154-68. doi: 10.4049/jimmunol.1000952. Epub 2010 Sep 8.

Abstract

CD18 integrins are adhesion molecules expressed on the cell surface of leukocytes and play a central role in the molecular mechanisms supporting leukocyte migration to zones of inflammation. Recently, it was discovered that CD11a/CD18 is shed from the leukocyte surface in models of inflammation. In this study, we show that shedding of human CD11/CD18 complexes is a part of synovial inflammation in rheumatoid arthritis and spondyloarthritis but not in osteoarthritis. In vivo and in vitro data suggest that the shedding is driven by TNF-α, which links the process to central events in the inflammatory response. The shed complexes contain multiple heterodimers of CD11/CD18, are variable in size, and differ according to the type of synovial inflammation. Furthermore, the differential structures determine the avidity of binding of the complexes to the ICAM-1. With the estimated concentrations of CD11/CD18 in plasma and synovial fluid a significant coverage of binding sites in ICAM-1 for CD18 integrins is expected. Based on cell adhesion experiments in vitro, we hypothesize that the large soluble complexes of CD11/CD18 act in vivo to buffer leukocyte adhesion by competing with the membrane-bound receptors for ICAM-1 binding sites. As reported here for synovial inflammation changes in the concentration or structure of these complexes should be considered as likely contributors to disease activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Arthritis / immunology
  • Arthritis / metabolism*
  • Arthritis / pathology
  • CD11 Antigens / immunology
  • CD11 Antigens / metabolism*
  • CD18 Antigens / immunology
  • CD18 Antigens / metabolism*
  • Cell Adhesion / immunology
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cell Separation
  • Epitopes / immunology
  • Female
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Humans
  • Inflammation / immunology
  • Inflammation / metabolism
  • Inflammation / pathology
  • Intercellular Adhesion Molecule-1 / immunology
  • Intercellular Adhesion Molecule-1 / metabolism
  • Leukocytes / immunology
  • Leukocytes / metabolism*
  • Male
  • Middle Aged
  • Multiprotein Complexes / immunology
  • Multiprotein Complexes / metabolism
  • Synovial Membrane / immunology*
  • Synovial Membrane / metabolism
  • Synovial Membrane / pathology

Substances

  • CD11 Antigens
  • CD18 Antigens
  • Epitopes
  • Multiprotein Complexes
  • Intercellular Adhesion Molecule-1