Energy-restricted high-fat diets only partially improve markers of systemic and adipose tissue inflammation

Obesity (Silver Spring). 2011 Feb;19(2):245-54. doi: 10.1038/oby.2010.196. Epub 2010 Sep 16.

Abstract

This study aimed at investigating whether the weight loss due to energy-restricted high-fat diets is accompanied with parallel improvements in metabolic markers and adipose tissue inflammation. Eight-week-old C57BL/6J mice were given free access to a low-fat (LF) or a high-fat (45% of energy from fat-HF) diet for 6 months. Restricting intake of the HF diet by 30% (HFR) during the last 2 months of the HF feeding trial decreased fasting plasma insulin, homeostasis model assessment of insulin resistance (HOMA(IR)), and plasma triglyceride levels and improved hepatic steatosis compared to ad libitum HF feeding, indicating an improved metabolic profile. Further, analysis of gonadal white adipose tissue (GWAT) gene expression by microarray and quantitative PCR analyses demonstrated that HFR downregulated expression of genes linked to cell and focal adhesion, cytokine-cytokine receptor interaction, and endoplasmic reticulum (ER)-associated degradation pathway. However, HFR had no effect on circulating plasminogen activator inhibitor-1 (PAI-1) and nonesterified fatty acid levels, which were persistently higher in both HF and HFR groups compared to the LF group. Furthermore, HFR had a negative effect on plasma total adiponectin level. Finally, while HFR decreased GWAT monocyte chemotactic protein-1 (MCP-1), interleukin-2 (IL-2), and PAI-1 levels, it did not affect several other cytokines including granulocyte-macrophage colony-stimulating factor, interferon-γ, IL-1β, IL-6, and IL-10. In summary, energy-restricted high-fat diets improve insulin sensitivity, while only partially improving markers of systemic and adipose tissue inflammation. In conclusion, our study supports the recommended low-fat intake for overall cardiovascular health.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adiponectin / metabolism
  • Adipose Tissue, White / metabolism*
  • Adipose Tissue, White / pathology
  • Animals
  • Biomarkers / metabolism
  • Blood Glucose / metabolism
  • Diabetes Mellitus / diet therapy
  • Diabetes Mellitus / metabolism
  • Diet, Reducing*
  • Dietary Fats / pharmacology*
  • Energy Intake / physiology*
  • Humans
  • Inflammation / metabolism*
  • Insulin / blood
  • Insulin Resistance
  • Lipids / blood
  • Male
  • Metabolic Syndrome / diet therapy*
  • Metabolic Syndrome / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Obesity / diet therapy
  • Obesity / metabolism
  • Triglycerides / blood

Substances

  • Adiponectin
  • Biomarkers
  • Blood Glucose
  • Dietary Fats
  • Insulin
  • Lipids
  • Triglycerides