An electrochemical supersandwich assay for sensitive and selective DNA detection in complex matrices

J Am Chem Soc. 2010 Oct 20;132(41):14346-8. doi: 10.1021/ja104998m.

Abstract

In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA / analysis*
  • Electrochemistry / methods*
  • Limit of Detection
  • Nucleic Acid Hybridization

Substances

  • DNA