Conformation-sensitive capillary electrophoresis

Methods Mol Biol. 2011:688:1-6. doi: 10.1007/978-1-60761-947-5_1.

Abstract

Conformation-sensitive capillary electrophoresis (CSCE) is a rapid, high-throughput screening method that can be applied to any region of a genome for detection of sequence variants. Slab gel-based conformation-sensitive gel electrophoresis was first described by Ganguly et al., and the transfer from slab gels to capillaries for higher throughput was reported by Rozycka et al. CSCE is based on the principle that DNA homoduplexes and heteroduplexes migrate at different rates during electrophoresis under mildly denaturing conditions. Fragments showing an altered peak morphology compared to the wild type are then sequenced to determine the precise nature of the sequence variant detected.

MeSH terms

  • DNA / chemistry*
  • DNA / genetics
  • DNA / isolation & purification*
  • DNA Primers / genetics
  • Electrophoresis, Capillary / methods*
  • Nucleic Acid Conformation*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide

Substances

  • DNA Primers
  • DNA