Toxicity and clearance of intratracheally administered multiwalled carbon nanotubes from murine lung

J Toxicol Environ Health A. 2010;73(21-22):1530-43. doi: 10.1080/15287394.2010.511578.

Abstract

Carbon nanotubes (CNT) are known to have widespread industrial applications; however, several reports indicated that these compounds may be associated with adverse effects in humans. In this study, multiwalled carbon nanotubes were administered to murine lungs intratracheally to determine whether acute and chronic pulmonary toxicity occurred. In particular, pristine multiwalled carbon nanotubes (PMWCNT) and acid-treated multiwalled carbon nanotubes (TMWCNT) were used in this study. In broncheoalveolar lavage fluid (BALF) cell analysis, PMWCNT induced more severe acute inflammatory cell recruitment than TMWCNT. Histopathologically, both PMWCNT and TMWCNT induced multifocal inflammatory granulomas in a dose-dependent manner. The observed granulomas were reversible, with TMWCNT-induced granulomas diminishing faster than PMWCNT-induced granulomas. Although the area of granuloma reduced with time, hyperplasia and dysplastic characteristics such as mitotic figures, anisokaryosis, and anisocytosis were still observed. These findings demonstrate that MWCNT induces granulomatous inflammation, and the duration and pattern of inflammation seem to vary depending upon the types of MWCNT to which mice are exposed. Therefore, toxicity studies on various types of CNT are needed as the responsiveness to these compounds differs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Air Pollutants / classification
  • Air Pollutants / pharmacokinetics
  • Air Pollutants / toxicity*
  • Animals
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / cytology
  • Chronic Disease
  • Dose-Response Relationship, Drug
  • Granuloma, Foreign-Body / chemically induced
  • Granuloma, Foreign-Body / pathology
  • Inhalation Exposure
  • Intubation, Intratracheal
  • Lung / drug effects*
  • Lung / pathology
  • Lung Diseases / chemically induced*
  • Lung Diseases / pathology
  • Male
  • Metabolic Clearance Rate
  • Mice
  • Mice, Inbred C57BL
  • Nanotubes, Carbon / chemistry
  • Nanotubes, Carbon / classification
  • Nanotubes, Carbon / toxicity*

Substances

  • Air Pollutants
  • Nanotubes, Carbon