Single-molecule four-color FRET

Angew Chem Int Ed Engl. 2010 Dec 17;49(51):9922-5. doi: 10.1002/anie.201005402.

Abstract

We developed a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopies. Real-time determination of six inter-fluorophore FRET efficiencies allowed us to probe the correlated motion of four arms of the Holliday junction. The technique was also applied to assess the correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Color
  • DNA, Cruciform / analysis*
  • Fluorescence Resonance Energy Transfer*
  • Microscopy, Fluorescence
  • Motion
  • Protein Conformation
  • Rec A Recombinases / analysis*

Substances

  • DNA, Cruciform
  • Rec A Recombinases