Abstract
The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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B-Lymphocytes / enzymology*
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Base Sequence
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Cell Line
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Genes, MHC Class II*
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Humans
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Immunologic Deficiency Syndromes / genetics*
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Immunologic Deficiency Syndromes / immunology
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Molecular Sequence Data
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Oligonucleotide Probes
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Polymerase Chain Reaction
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RNA Precursors / genetics
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RNA, Messenger / genetics
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Reference Values
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Transcription, Genetic*
Substances
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Oligonucleotide Probes
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RNA Precursors
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RNA, Messenger