Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction

J Virol Methods. 2011 Mar;172(1-2):78-80. doi: 10.1016/j.jviromet.2010.12.006. Epub 2010 Dec 21.

Abstract

The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • HIV Infections / virology*
  • HIV-1 / genetics
  • HIV-1 / isolation & purification
  • HIV-1 / physiology*
  • Humans
  • RNA, Viral / blood
  • RNA, Viral / genetics
  • Reagent Kits, Diagnostic
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Load / methods*

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic