Evaluation of Salmonella-vectored Campylobacter peptide epitopes for reduction of Campylobacter jejuni in broiler chickens

Clin Vaccine Immunol. 2011 Mar;18(3):449-54. doi: 10.1128/CVI.00379-10. Epub 2010 Dec 22.

Abstract

Campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. Live Salmonella vectors expressing three linear peptide epitopes from Campylobacter proteins Cj0113 (Omp18/CjaD), Cj0982c (CjaA), and Cj0420 (ACE393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged with Campylobacter jejuni on day 21. All three candidate vaccines produced consistent humoral immune responses with high levels of serum IgG and mucosal secretory IgA (sIgA), with the best response from the Cj0113 peptide-expressing vector. Campylobacter challenge following vaccination of three candidate vaccine groups decreased Campylobacter recovery from the ileum compared to that for controls on day 32. The Cj0113 peptide-expressing vector reduced Campylobacter to below detectable levels. The Salmonella-vectored Cj0113 subunit vaccine appears to be an excellent candidate for further evaluation as a tool for the reduction of Campylobacter in poultry for improved food safety.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies, Bacterial / analysis
  • Antibodies, Bacterial / blood
  • Bacterial Load
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / immunology*
  • Campylobacter Infections / prevention & control*
  • Campylobacter jejuni / immunology*
  • Campylobacter jejuni / isolation & purification
  • Carrier State / prevention & control*
  • Chickens
  • Drug Carriers*
  • Epitopes / genetics
  • Epitopes / immunology
  • Genetic Vectors
  • Ileum / microbiology
  • Immunoglobulin A / analysis
  • Immunoglobulin G / blood
  • Intestinal Mucosa / immunology
  • Salmonella / genetics*
  • Serum / immunology
  • Vaccines, Subunit / administration & dosage
  • Vaccines, Subunit / genetics
  • Vaccines, Subunit / immunology

Substances

  • Antibodies, Bacterial
  • Bacterial Proteins
  • Bacterial Vaccines
  • Drug Carriers
  • Epitopes
  • Immunoglobulin A
  • Immunoglobulin G
  • Vaccines, Subunit