We surveyed populations of the broad-snouted caiman, Caiman latirostris, throughout the São Francisco River basin, from Três Marias reservoir, State of Minas Gerais, to the river delta, at the boarder of Sergipe and Alagoas states. We registered the occurrence of crocodilians in 61% of all surveyed localities (n = 64), in which the presence of C. latirostris was confirmed in 44% of the surveyed sites. Caimans occurred in both lentic and lotic habitats, although there was a preference for small dams, oxbow lakes and wetlands. Despite the hunting pressure and human impact on natural habitats, our results indicate that the populations of C. latirostris in the São Francisco basin are not fragmented.