Localization of antimicrobial peptides on polymerized liposomes leading to their enhanced efficacy against Pseudomonas aeruginosa

Mol Biosyst. 2011 Mar;7(3):711-3. doi: 10.1039/c0mb00207k. Epub 2011 Jan 13.

Abstract

Antimicrobial peptide IG-25 (a truncated version of LL-37 of the cathelicidin family) tethering an azido-capped poly(ethylene glycol) chain at the N-terminus was site-specifically attached to alkynyl-terminated polymerized liposomes using copper catalyzed "click" reaction, leading to an 18 fold enhancement in efficacy against Pseudomonas aeruginosa when compared to LL-37 without any increase in cytotoxicity to human corneal epithelial cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Antimicrobial Cationic Peptides / chemistry
  • Antimicrobial Cationic Peptides / pharmacology*
  • Catalysis
  • Cathelicidins
  • Click Chemistry
  • Copper / chemistry
  • Cornea / cytology
  • Cornea / drug effects
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Humans
  • Liposomes / chemical synthesis
  • Liposomes / chemistry*
  • Microbial Sensitivity Tests
  • Molecular Structure
  • Polyethylene Glycols / chemistry*
  • Pseudomonas aeruginosa / drug effects*
  • Structure-Activity Relationship

Substances

  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • I-G-K-E-F-K-R-I-V-Q-R-I-K-D-F-L-R-N-L-V-P-R-T-E-S
  • Liposomes
  • Polyethylene Glycols
  • Copper
  • Cathelicidins