Molecular phylogenetic analysis of Enterobius vermicularis and development of an 18S ribosomal DNA-targeted diagnostic PCR

J Clin Microbiol. 2011 Apr;49(4):1602-4. doi: 10.1128/JCM.02454-10. Epub 2011 Jan 19.

Abstract

We genetically characterized pinworms obtained from 37 children from different regions of Germany and established new species-specific molecular diagnostic tools. No ribosomal DNA diversity was found; the phylogenetic position of Enterobius vermicularis within the Oxyurida order and its close relationship to the Ascaridida and Spirurida orders was confirmed.

MeSH terms

  • Animals
  • Child
  • Child, Preschool
  • Cluster Analysis
  • DNA Primers / genetics
  • DNA, Helminth / chemistry
  • DNA, Helminth / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Enterobiasis / diagnosis*
  • Enterobiasis / parasitology
  • Enterobius / genetics*
  • Enterobius / isolation & purification*
  • Germany
  • Humans
  • Molecular Sequence Data
  • Parasitology / methods*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 18S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA, Helminth
  • DNA, Ribosomal
  • RNA, Ribosomal, 18S

Associated data

  • GENBANK/HQ646164