[Evaluation of the SD BIOLINE TB Ag MPT64 Rapid® for the diagnosis of tuberculosis]

Pathol Biol (Paris). 2011 Feb;59(1):26-8. doi: 10.1016/j.patbio.2010.08.007. Epub 2011 Jan 31.
[Article in French]

Abstract

The purpose of this study was to evaluate the SD Bioline Ag MPT64 Rapid(®) for identification of the Mycobacterium tuberculosis complex. The method uses an immunochromatographic assay and needs 100 μl of sample taken from liquid culture or colonies suspended. The sensitivity was determined using 99 strains of M. tuberculosis complex and the specificity using 10 nontuberculous mycobacteria and 85 strains other than mycobacteria genus. The test showed excellent sensitivity (99%) and specificity (100%). This technique displays several advantages and is destined to spread in all laboratories and particularly in endemic areas.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Bacterial / immunology
  • Antibodies, Immobilized
  • Antigens, Bacterial / analysis*
  • Antigens, Bacterial / genetics
  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics
  • Chromatography / methods*
  • Enterobacteriaceae / immunology
  • False Positive Reactions
  • Female
  • Gram-Positive Bacteria / immunology
  • Humans
  • Immunoblotting / methods*
  • In Vitro Techniques
  • Male
  • Mutation
  • Mycobacterium / genetics
  • Mycobacterium / immunology
  • Mycobacterium / isolation & purification
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / immunology*
  • Mycobacterium tuberculosis / isolation & purification
  • Sensitivity and Specificity
  • Species Specificity
  • Suspensions
  • Time Factors
  • Tuberculosis / diagnosis*

Substances

  • Antibodies, Bacterial
  • Antibodies, Immobilized
  • Antigens, Bacterial
  • Bacterial Proteins
  • MPB64 protein, Mycobacterium
  • MPT64 protein, Mycobacterium tuberculosis
  • Suspensions