Octreotide lessens peritoneal injury in experimental encapsulated peritoneal sclerosis model

Nephrology (Carlton). 2011 Aug;16(6):552-7. doi: 10.1111/j.1440-1797.2011.01460.x.

Abstract

Aim: Encapsulated peritoneal sclerosis is characterized by neoangiogenesis and fibrosis. Octreotide, a somatostatin analogue is a well-known antifibrotic, antiproliferative and anti-angiogenic agent. The aim of the study is to evaluate the effects of octreotide in encapsulated peritoneal sclerosis-induced neoangiogenesis and fibrosis and compare the results with resting.

Methods: Non-uraemic Wistar-Albino male rats (n = 35) were divided into four groups. Group I, control rats, received 2 mL isotonic saline i.p. daily for 3 weeks. Group II, received daily i.p. 2 mL/200 g injection of chlorhexidine gluconate (0.1%) and ethanol (%15) dissolved in saline for 3 weeks. Group III, chlorhexidine gluconate for 3 weeks plus an additional 3 weeks without any treatment (rest), to a total of 6 weeks. Group IV, chlorhexidine gluconate for 3 weeks plus an additional 3 weeks octreotide, 50 mcg/kg bodyweight s.c., for a total of 6 weeks.

Results: Octreotide significantly reversed ultrafiltration capacity of peritoneum with decreasing inflammation, neoangiogenesis and fibrosis compared to the resting group. Octreotide also caused inhibition of dialysate transforming growth factor-β1, vascular endothelial growth factor and monocyte chemotactic protein-1 activity and improved mesothelial cell cytokeratin expression. Peritoneal resting has no beneficial effects on peritoneum.

Conclusion: In conclusion, octreotide may have a therapeutic value in peritoneal dialysis patients who suffer from encapsulated peritoneal sclerosis.

MeSH terms

  • Angiogenesis Inhibitors / pharmacology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Chemokine CCL2 / metabolism
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / analogs & derivatives
  • Chlorhexidine / metabolism
  • Dialysis Solutions / administration & dosage
  • Dialysis Solutions / metabolism
  • Disease Models, Animal
  • Fibrosis
  • Male
  • Neovascularization, Pathologic / drug therapy*
  • Neovascularization, Pathologic / metabolism
  • Neovascularization, Pathologic / pathology
  • Octreotide / pharmacology*
  • Peritoneal Dialysis / adverse effects*
  • Peritoneal Fibrosis / drug therapy*
  • Peritoneal Fibrosis / etiology
  • Peritoneal Fibrosis / metabolism
  • Peritoneal Fibrosis / pathology
  • Peritoneum / drug effects*
  • Peritoneum / metabolism
  • Peritoneum / pathology
  • Permeability
  • Rats
  • Rats, Wistar
  • Transforming Growth Factor beta1 / metabolism
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Angiogenesis Inhibitors
  • Anti-Inflammatory Agents
  • Ccl2 protein, rat
  • Chemokine CCL2
  • Dialysis Solutions
  • Tgfb1 protein, rat
  • Transforming Growth Factor beta1
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • chlorhexidine gluconate
  • Chlorhexidine
  • Octreotide