Edible neotropical blueberries: antioxidant and compositional fingerprint analysis

J Agric Food Chem. 2011 Apr 13;59(7):3020-6. doi: 10.1021/jf200367j. Epub 2011 Mar 10.

Abstract

Edible blueberry species are well recognized for their potential health benefits. Ericaceae fruits including the North American highbush blueberry (Vaccinium corymbosum L.) and five less common edible blueberry relatives from the New World tropics, Anthopterus wardii Ball, Cavendishia grandifolia Hoerold, Macleania coccoloboides A. C. Smith, Sphyrospermum buxifolium Poeppig & Endlicher, and Sphyrospermum cordifolium Benth, were investigated for their antioxidant properties and phenolic profiles. The neotropical berries C. grandifolia and A. wardii exhibited significantly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS(• +)) free radical scavenging and iron chelation activities than V. corymbosum. Total phenolic content and high-performance liquid chromatography with photodiode array detection (HPLC-PDA) compositional fingerprint analyses were also carried out. Significant correlations were observed among total phenolic contents, DPPH(•) and ABTS(• +) scavenging, and iron chelation activities. By use of HPLC-PDA, the phenolic constituents in the berries were identified as chlorogenic acid, p-coumaric acid, hyperoside, quercetin-3-O-glucoside, isoorientin, isovitexin, orientin and vitexin. Principal component analysis reduced the dimensions of antioxidant and total phenolic data to two components, which accounted for 95% of total variation among the six fruits. Each fruit species formed its own cluster, and therefore the antioxidant profile of each species was shown to be distinct.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Anthocyanins / analysis
  • Antioxidants / analysis*
  • Benzothiazoles
  • Biphenyl Compounds
  • Blueberry Plants / chemistry*
  • Chromatography, High Pressure Liquid
  • Ericaceae / chemistry*
  • Flavonoids / analysis
  • Fruit / chemistry*
  • Phenols / analysis*
  • Picrates
  • Sulfonic Acids
  • Tropical Climate

Substances

  • Anthocyanins
  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Flavonoids
  • Phenols
  • Picrates
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • 1,1-diphenyl-2-picrylhydrazyl