Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k

Phys Rev Lett. 2011 Feb 18;106(7):078101. doi: 10.1103/PhysRevLett.106.078101. Epub 2011 Feb 15.

Abstract

The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10(-3) s(-1) around hydrogen bonds at the flavin cofactor binding site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Euglena gracilis
  • Flavins / metabolism*
  • Flavoproteins / chemistry*
  • Flavoproteins / metabolism*
  • Hydrogen Bonding
  • Models, Molecular
  • Photons
  • Protein Structure, Tertiary
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / metabolism
  • Spectrometry, Fluorescence / methods*
  • Temperature*

Substances

  • Flavins
  • Flavoproteins
  • Protozoan Proteins