Engineering the ABA plant stress pathway for regulation of induced proximity

Sci Signal. 2011 Mar 15;4(164):rs2. doi: 10.1126/scisignal.2001449.

Abstract

Chemically induced proximity (CIP) systems use small molecules and engineered proteins to control and study biological processes. However, small molecule-based systems for controlling protein abundance or activities have been limited by toxicity, instability, cost, and slow clearance of the small molecules in vivo. To address these problems, we modified proteins of the plant abscisic acid (ABA) stress response pathway to control the proximity of cellular proteins and showed that the system could be used to regulate transcription, signal transduction, and subcellular localization of proteins in response to exogenously applied ABA. We also showed that the ABA CIP system can be combined with other CIP systems to simultaneously control multiple processes. We found that, when given to mice, ABA was orally available and had a 4-hour half-life. These properties, along with its lack of toxicity and low cost, suggest that ABA may be well suited for therapeutic applications and as an experimental tool to control diverse cellular activities in vivo.

MeSH terms

  • Abscisic Acid / metabolism*
  • Abscisic Acid / pharmacokinetics
  • Abscisic Acid / pharmacology*
  • Abscisic Acid / toxicity
  • Amino Acid Sequence
  • Animals
  • Arabidopsis / metabolism*
  • Base Sequence
  • Bioengineering
  • Biological Availability
  • Cell Line
  • DNA Primers / genetics
  • Drug Stability
  • Gene Expression / drug effects
  • Humans
  • Mice
  • Molecular Sequence Data
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction / drug effects
  • Sirolimus / pharmacology
  • Stress, Physiological
  • Transfection

Substances

  • DNA Primers
  • Plant Growth Regulators
  • Recombinant Fusion Proteins
  • Abscisic Acid
  • Sirolimus