Piglet model for studying esophageal regrowth after resection and interposition of a silicone stented small intestinal submucosa tube

Eur Surg Res. 2011;46(4):169-79. doi: 10.1159/000324401. Epub 2011 Mar 31.

Abstract

Purpose: To investigate the use and subsequent healing of a silicone stented small intestinal submucosa (SIS) tube as a full-circumference replacement following surgical resection of the esophagus in piglets.

Material and methods: Three centimeters of the intrathoracic esophagus was replaced with a silicone stented SIS tube (Cook Medical) in 6 growing piglets. The esophageal stent was retained for 4 weeks. Esophageal dilations were performed, if needed, after stent removal.

Results: The piglets were sacrificed 1-17 weeks after surgery. Recurrent dilations were needed after stent removal. Histology showed that the gap between the resection margins was filled with new loose connective tissue consisting of fibroblasts and few inflammatory cells. In this tissue, intense angiogenesis was seen at the early time points, which then gave way to the proliferation of immature-looking smooth-muscle-like cells in the submucosa, which appeared to stem from the pericytes of the ingrowing capillaries.

Conclusions: Through using a stented SIS tube as a circumferential esophageal replacement in a piglet model, this study suggests that pericytes from ingrowing capillaries may play a role in the remodeling of the SIS mesh. It remains to be seen if this process gives a favorable end result because stricture formation after stent removal remains a problem.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Esophagoplasty / methods*
  • Esophagus / physiology*
  • Esophagus / surgery*
  • Esophagus / ultrastructure
  • Intestinal Mucosa / transplantation*
  • Intestine, Small / transplantation
  • Microscopy, Electron
  • Models, Animal
  • Regeneration
  • Silicones
  • Stents*
  • Swine
  • Wound Healing*

Substances

  • Silicones