Abstract
Here we report a comprehensive biological characterization of a potent and selective small-molecule inhibitor of the DNA damage response (DDR) kinase ATR. We show a profound synthetic lethal interaction between ATR and the ATM-p53 tumor suppressor pathway in cells treated with DNA-damaging agents and establish ATR inhibition as a way to transform the outcome for patients with cancer treated with ionizing radiation or genotoxic drugs.
MeSH terms
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Animals
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacology*
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Ataxia Telangiectasia Mutated Proteins
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Cell Cycle Proteins / antagonists & inhibitors*
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Cell Cycle Proteins / genetics
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Cell Death / drug effects
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Cell Death / genetics
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Cell Line, Tumor
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Cell Proliferation / drug effects
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DNA Damage
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DNA-Binding Proteins / deficiency*
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DNA-Binding Proteins / genetics
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Dose-Response Relationship, Drug
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Humans
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Molecular Structure
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Protein Serine-Threonine Kinases / antagonists & inhibitors*
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Protein Serine-Threonine Kinases / deficiency*
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Protein Serine-Threonine Kinases / genetics
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Pyrazines / chemistry
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Pyrazines / pharmacology*
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Sulfones / chemistry
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Sulfones / pharmacology*
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Tumor Suppressor Protein p53 / deficiency*
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Proteins / deficiency*
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Tumor Suppressor Proteins / genetics
Substances
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3-amino-6-(4-(methylsulfonyl)phenyl)-N-phenylpyrazine-2-carboxamide
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Antineoplastic Agents
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Cell Cycle Proteins
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DNA-Binding Proteins
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Pyrazines
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Sulfones
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Tumor Suppressor Protein p53
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Tumor Suppressor Proteins
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ATM protein, human
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ATR protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases
Associated data
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PubChem-Substance/117682505
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PubChem-Substance/117682506
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PubChem-Substance/117682507
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PubChem-Substance/117682508
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PubChem-Substance/117682509
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PubChem-Substance/117682510
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PubChem-Substance/117682511
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PubChem-Substance/117682512
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PubChem-Substance/117682513
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PubChem-Substance/117682514
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PubChem-Substance/117682515
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PubChem-Substance/117682516
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PubChem-Substance/117682517
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PubChem-Substance/117682518
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PubChem-Substance/117682519
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PubChem-Substance/117682520