Development of 14-epi-19-nortachysterol and its unprecedented binding configuration for the human vitamin D receptor

J Am Chem Soc. 2011 May 11;133(18):7215-21. doi: 10.1021/ja201481j. Epub 2011 Apr 18.

Abstract

In the study of the synthesis of 14-epi-19-norprevitamin D(3), we found 14-epi-19-nortachysterol derivatives through C6,7-cis/trans isomerization. We also succeeded in their chemical synthesis and revealed their marked stability and potent VDR binding affinity. To the best of our knowledge, this is the first isolation of stable tachysterol analogues. Surprisingly, 14-epi-19-nortachysterol derivatives exhibited an unprecedented binding configurations for the ligand binding pocket in hVDR, C5,6-s-trans and C7,8-s-trans triene configurations, which were opposite the natural C7,8-ene-configuration of 1α,25(OH)(2)D(3).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholecalciferol / analogs & derivatives
  • Cholecalciferol / chemistry
  • Crystallography, X-Ray
  • Humans
  • Isomerism
  • Ligands
  • Molecular Structure
  • Protein Binding
  • Receptors, Calcitriol / chemistry*

Substances

  • Ligands
  • Receptors, Calcitriol
  • Cholecalciferol
  • previtamin D(3)

Associated data

  • PDB/3AUQ
  • PDB/3AUR