STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution

Mike Friedrich; Qiang Gan; Vladimir Ermolayev; Gregory S Harms

doi:10.1016/j.bpj.2010.12.3748

STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution

Biophys J. 2011 Apr 20;100(8):L43-5. doi: 10.1016/j.bpj.2010.12.3748.

Authors

Mike Friedrich¹, Qiang Gan, Vladimir Ermolayev, Gregory S Harms

Affiliation

¹ Molecular Microscopy Group, Bio-Imaging Center, Rudolf Virchow Center, University of Würzburg, Würzburg, Germany.

Abstract

We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 μm deep penetration into biological tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Embryo, Nonmammalian
Lighting / methods*
Microscopy / methods*
Optical Phenomena
Zebrafish / embryology