Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen; Lisbeth Nielsen Fink; Carsten Struve; Claus Sternberg; Jens Bo Andersen; Jørn Brynskov; Ole Haagen Nielsen; Susanne Brix

doi:10.1016/j.mimet.2011.04.002

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

J Microbiol Methods. 2011 Jul;86(1):111-4. doi: 10.1016/j.mimet.2011.04.002. Epub 2011 Apr 12.

Authors

Stina Rikke Jensen¹, Lisbeth Nielsen Fink, Carsten Struve, Claus Sternberg, Jens Bo Andersen, Jørn Brynskov, Ole Haagen Nielsen, Susanne Brix

Affiliation

¹ Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. SJEN0236@heh.regionh.dk

PMID: 21504765
DOI: 10.1016/j.mimet.2011.04.002

Abstract

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Crohn Disease / microbiology*
DNA Primers / genetics
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Humans
Intestinal Mucosa / microbiology*
Polymerase Chain Reaction / methods*

Substances

DNA Primers