Murine cerebral malaria: histopathology and ICAM 1 immunohistochemistry of the inner ear

Trop Med Int Health. 2011 Aug;16(8):914-22. doi: 10.1111/j.1365-3156.2011.02801.x. Epub 2011 May 9.

Abstract

Objective: To evaluate the pathophysiologic changes in the inner ear during the course of severe cerebral malaria in an established animal model, C57 BL/6J mice.

Methods: This study aims to examine the hearing threshold, the histological changes and ICAM-1 expression in the murine cochlea.

Results: Four of seven mice showed an expected hearing loss of 20 dB or more. The light microscopy of the inner ear did not show any morphologic alterations. The immunohistochemical analysis for ICAM-1 showed intensive staining in the stria vascularis of sick animals and hardly any reaction in healthy controls.

Conclusion: The up-regulation of ICAM-1 in the stria vascularis - generating the endocochlear potential - suggests its involvement in plasmodial infection.

MeSH terms

  • Animals
  • Evoked Potentials, Auditory, Brain Stem / physiology
  • Hearing Loss / metabolism*
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Malaria, Cerebral / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Plasmodium berghei
  • Stria Vascularis / metabolism*

Substances

  • Intercellular Adhesion Molecule-1